Recombinant Mouse Klk1 protein, His & GST-tagged

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Recombinant Mouse Klk1 protein, His & GST-tagged

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Cat.No. : Klk1-5678M
Product Overview : Recombinant Mouse Klk1 aa. (Gly27~Asp261) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. Mice lacking the encoded protein are unable to generate significant levels of kinins in most tissues, develop cardiovascular abnormalities and exhibit hypercalciuria of renal origin. This gene is located in a cluster of several related kallikrein genes on chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing.
Source : E. coli
Species : Mouse
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : 56kDa as determined by SDS-PAGE reducing conditions.
Protein length : Gly27~Asp261
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >80%
Characteristic : The isoelectric point is 5.7.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : Klk1 kallikrein 1 [ Mus musculus (house mouse) ]
Official Symbol : Klk1
Synonyms : Klk1; kallikrein 1; Kal; Klk6; KAL-B; mGk-6; Klk1b6; 0610007D04Rik; kallikrein-1; glandular kallikrein K1; kallikrein renal/pancreas/salivary; tissue kallikrein-6; true tissue kallikrein
Gene ID : 16612
mRNA Refseq : NM_001320331.1
Protein Refseq : NP_001307260.1
UniProt ID : P15947

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