Active Recombinant Human KLRK1 protein, lFc-tagged

• Cat.No.: KLRK1-0580H
• Product Overview: Recombinant Human KLRK1 protein(Ile73-Val216), fused with lFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: lFc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human MICA, His Tag at 5 μg/mL (100 μL/well) can bind Human NKG2D, Llama IgG2b Fc Tag with a linear range of 0.01-0.625 μg/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
• Molecular Mass: This protein carries a llama IgG2b Fc tag at the N-terminus.The protein has a calculated MW of 44.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ile73-Val216
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
• Official Symbol: KLRK1
• Synonyms: KLRK1; killer cell lectin-like receptor subfamily K, member 1; D12S2489E, DNA segment on chromosome 12 (unique) 2489 expressed sequence; NKG2-D type II integral membrane protein; CD314; KLR; NKG2 D; NKG2D; NK cell receptor D; NKG2-D-activating NK receptor; NKG2-D; D12S2489E; FLJ17759; FLJ75772
• Gene ID: 22914
• mRNA Refseq: NM_007360
• Protein Refseq: NP_031386
• MIM: 611817
• UniProt ID: P26718