Active Recombinant Human KNG1 protein, His-tagged

• Cat.No.: KNG1-3179H
• Product Overview: Active Recombinant Human KNG1 protein(NP_001095886.1) (Gln 19-Ser 644) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus, and a signal peptide at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-644 aa
• Form: Lyophilized from sterile 25mM Tris, 100mM NaCl, pH 7.5.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is < 7 nM.
• Molecular Mass: The recombinant human KNG1 consists of 637 amino acids and has a predicted molecular mass of 71.3 kDa. As a result of glycosylation, the apparent molecular mass of rhKNG1 is approximately 100-110 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KNG1 kininogen 1 [ Homo sapiens ]
• Official Symbol: KNG1
• Synonyms: KNG1; kininogen 1; BDK, kininogen , KNG; kininogen-1; alpha 2 thiol proteinase inhibitor; bradykinin; HMWK; fitzgerald factor; high molecular weight kininogen; alpha-2-thiol proteinase inhibitor; williams-Fitzgerald-Flaujeac factor; BDK; KNG
• Gene ID: 3827
• mRNA Refseq: NM_000893
• Protein Refseq: NP_000884
• MIM: 612358
• UniProt ID: P01042