Active Recombinant Human L1CAM Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: L1CAM-485HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human L1CAM (Accession # CAA42508) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. When 5 × 10^4 cells/well are added to recombinant Human L1CAM Fc Chimera coated plates (25 μg/mL with 100 μL/well), > 30% will adhere for 1 hour incubation at 37 centigrade. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human L1CAM, Fc Chimera has a calculated MW of 150.4 kDa (monomer) & 55.9 kDa (truncated). In SDS-PAGE migrates as 210-225 kDa, reducing conditions.
• N-terminal Sequence Analysis: Ile 20 & Arg 864
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens ]
• Official Symbol: L1CAM
• Synonyms: L1CAM; L1 cell adhesion molecule; antigen identified by monoclonal R1 , HSAS, HSAS1, MASA, MIC5, S10, SPG1; neural cell adhesion molecule L1; CD171; antigen identified by monoclonal R1; S10; HSAS; MASA; MIC5; SPG1; CAML1; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Gene ID: 3897
• mRNA Refseq: NM_000425
• Protein Refseq: NP_000416
• MIM: 308840
• UniProt ID: P32004

Reviews

04/11/2020

L1CAM protein is an enzyme involved in the digestion of carbohydrates, specifically starch.

07/07/2019

A reliable manufacturer ensures the production and purification of L1CAM protein, maintaining its quality and purity.

10/08/2017

Manufacturers should provide detailed information about the production process, quality control measures, and purity assessment methods employed.

Q&As

Are there any L1CAM-targeted therapies in clinical trials?

Some clinical trials are exploring L1CAM-targeted therapies in the context of cancer treatment.

Can L1CAM be used as a diagnostic marker for neurological disorders?

L1CAM mutations can be diagnosed through genetic testing, aiding in the diagnosis of L1 syndrome and related conditions.

Can L1CAM be a therapeutic target in cancer treatment?

L1CAM has been explored as a potential therapeutic target for inhibiting tumor metastasis in some cancer types.

Are there clinical applications for L1CAM in neurodegenerative diseases?

L1CAM is not typically associated with neurodegenerative diseases, but its role in neural development may have implications for regenerative medicine.

In what other medical conditions is L1CAM implicated?

L1CAM has been associated with certain developmental disorders and cognitive deficits in addition to cancer and neurological disorders.