Active Recombinant Human L1CAM protein, His-tagged

• Cat.No.: L1CAM-02H
• Product Overview: Recombinant human L1CAM, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a mouse neuroblastoma cells. When cells are added to human L1CAM coated plates 1 μg/mL. This effect is more to 30 %.
• Molecular Mass: 123.6 kDa (1104aa)
• Protein length: 20-1115
• AA Sequence: IQIPEELMEPPVITEQSPRRLVVFP TDDISLKCEASGKPEVQFRWTRDGV HFKPKEELGVTVYQSPHSGSFTITG NNSNFAQRFQGIYRCFASNKLGTAM SHEIRLMAEGAPKWPKETVKPVEVE EGESVVLPCNPPPSAEPLRIYWMNS KILHIKQDERVTMGQNGNLYFANVL TSDNHSDYICHAHFPGTRTIIQKEP IDLRVKATNSMIDRKPRLLFPTNSS SHLVALQGQPLVLECIAEGFPTPTI KWLRPSGPMPADRVTYQNHNKTLQL LKVGEEDDGEYRCLAENSLGSARHA YYVTVEAAPYWLHKPQSHLYGPGET ARLDCQVQGRPQPEVTWRINGIPVE ELAKDQKYRIQRGALILSNVQPSDT MVTQCEARNRHGLLLANAYIYVVQL PAKILTADNQTYMAVQGSTAYLLCK AFGAPVPSVQWLDEDGTTVLQDERF FPYANGTLGIRDLQANDTGRYFCLA ANDQNNVTIMANLKVKDATQITQGP RSTIEKKGSRVTFTCQASFDPSLQP SITWRGDGRDLQELGDSDKYFIEDG RLVIHSLDYSDQGNYSCVASTELDV VESRAQLLVVGSPGPVPRLVLSDLH LLTQSQVRVSWSPAEDHNAPIEKYD IEFEDKEMAPEKWYSLGKVPGNQTS TTLKLSPYVHYTFRVTAINKYGPGE PSPVSETVVTPEAAPEKNPVDVKGE GNETTNMVITWKPLRWMDWNAPQVQ YRVQWRPQGTRGPWQEQIVSDPFLV VSNTSTFVPYEIKVQAVNSQGKGPE PQVTIGYSGEDYPQAIPELEGIEIL NSSAVLVKWRPVDLAQVKGHLRGYN VTYWREGSQRKHSKRHIHKDHVVVP ANTTSVILSGLRPYSSYHLEVQAFN GRGSGPASEFTFSTPEGVPGHPEAL HLECQSNTSLLLRWQPPLSHNGVLT GYVLSYHPLDEGGKGQLSFNLRDPE LRTHNLTDLSPHLRYRFQLQATTKE GPGEAIVREGGTMALSGISDFGNIS ATAGENYSVVSWVPKEGQCNFRFHI LFKALGEEKGGASLSPQYVSYNQSS YTQWDLQPDTDYEIHLFKERMFRHQ MAVKTNGTGRVRLPPAGFATE
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90 % by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL
• Storage Buffer: PBS (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens (human) ]
• Official Symbol: L1CAM
• Synonyms: L1CAM; L1 cell adhesion molecule; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1; neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
• Gene ID: 3897
• mRNA Refseq: NM_001143963
• Protein Refseq: NP_001137435
• MIM: 308840
• UniProt ID: P32004