Active Recombinant Human LCK protein, GST-tagged

• Cat.No.: LCK-6530H
• Product Overview: Active Recombinant Human LCK protein(NP_001036236.1) (Met 1-Pro 509) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-509 aa
• Form: Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM GSH, 0.1mM EGTA, 0.1mM EDTA, 0.5mM PMSF, 10% glycerol.
• Bio-activity: 1. The specific activity was determined to be 70 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate. 2. Measured by its ability to bind recombinant human CD3E in a functional ELISA.
• Molecular Mass: The recombinant human LCK/GST chimera consists of 734 amino acids and predicts a molecular mass of 84.4 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.2-1.0 mg/mL.

Gene Information

• Gene Name: LCK lymphocyte-specific protein tyrosine kinase [ Homo sapiens ]
• Official Symbol: LCK
• Synonyms: LCK; lymphocyte-specific protein tyrosine kinase; tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; LSK; YT16; p56lck; pp58lck
• Gene ID: 3932
• mRNA Refseq: NM_001042771
• Protein Refseq: NP_001036236
• MIM: 153390
• UniProt ID: P06239