Active Recombinant Human Leucine Rich Repeat Neuronal 3, His-tagged

• Cat.No.: LRRN3-5681H
• Product Overview: Recombinant Human LRRN3protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Val23-Thr628.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 23-628 a.a.
• Description: LRRN3 is an 85 kDa,N-glycosylated member of a CNS-associated family of transmembrane moleculesthat includes AMIGO, FLRT, LINGO, LRIG, SLITRK, and Trk subgroups. Maturehuman LRRN3 consists of a 606 amino acid extracellular domain with 13 LRRs,an RGD integrin-binding motif, one Ig-like domain, and one fibronectin typeIII-like domain, a 21 aa transmembrane segment, and a 59 aa cytoplasmicdomain. Within the ECD, human LRRN3 shares 91% aa sequence identity withmouse and rat LRRN3.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Val23
• Molecular Weight: 69.3 kDa
• Activity: Measuredby the ability of the immobilized protein to enhance the adhesion of SAOS2human osteosarcoma cells to human Fibronectin. When 3 x 104 cellsper well are added to rhLRRN3 coated plates (5 μg/mL, 100 μL/well),approximately 50-70% will adhere after 1 hour at 37°C.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 90-95kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 200μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: LRRN3 leucine rich repeatneuronal 3 [ Homo sapiens ]
• Official Symbol: LRRN3
• Synonyms: LRRN3; leucine richrepeat neuronal 3; NLRR3; NLRR-3; FIGLER5; FLJ11129; leucine-rich repeatneuronal protein 3; neuronal leucine-rich repeat protein 3; leucine-richrepeat protein, neuronal 3; fibronectin type III, immunoglobulin and leucinerich repeat domains 5; Nbla10363; UNQ194/PRO220; OTTHUMP00000207165;OTTHUMP00000207166; OTTHUMP00000207167
• Gene ID: 54674
• mRNA Refseq: NM_001099660
• Protein Refseq: NP_001093130
• UniProt ID: Q9H3W5
• Chromosome Location: 7q31.1
• Function: protein complexbinding