| Species : |
Human |
| Source : |
CHO |
| Tag : |
Fc |
| Description : |
The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body. |
| Form : |
Disulfide-linked homodimer, Lyophilized from a 0.2 μm filtered solution in PBS. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human R-Spondin 3 is coated at 0.5 μg/mL, Recombinant Human Lgr4/GPR48 Fc Chimera binds with an apparent KD <10 nM. |
| Molecular Mass : |
83.8 kDa (monomer) |
| Endotoxin : |
< 0.10 EU/μg of the protein by the LAL method. |
| Purity : |
> 90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Shipping : |
The product is shipped at ambient temperature. |
| Reconstitution : |
Reconstitute at 200 μg/mL in PBS. |
| N-terminal Sequence Analysis : |
Ala25 |
| Reference : |
1. Loh, E.D. et al. (2001) Biochem. Biophys. Res. Commun. 282:757.
2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452. |
