Active Recombinant Human LIFR protein(Met1-Ser833), His-tagged

• Cat.No.: LIFR-611H
• Product Overview: Recombinant Human LIFR (NP_001121143.1) (Met 1-Ser 833) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Ser833
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized LIF Protein, Human, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind LIFR Protein, Human, Recombinant (His Tag), the EC50 is 60-360 ng/mL.
• Molecular Mass: The secreted recombinant human LIFR comprises 800 amino acids with a predicted molecular mass of 91 kDa. As a result of glycosylation, rhLIFR migrates as an approximately 125-135 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LIFR leukemia inhibitory factor receptor alpha [ Homo sapiens ]
• Official Symbol: LIFR
• Synonyms: LIFR; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; CD118; LIF receptor; CD118 antigen; SWS; SJS2; STWS; LIF-R; FLJ98106; FLJ99923
• Gene ID: 3977
• mRNA Refseq: NM_001127671
• Protein Refseq: NP_001121143
• MIM: 151443
• UniProt ID: P42702