Recombinant Human MADCAM1 protein, His-tagged

• Cat.No.: MADCAM1-3982H
• Product Overview: Recombinant Human MADCAM1 protein(Q13477-1)(Gln19-Gln317), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln19-Gln317
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human MADCAM1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
• Gene ID: 8174
• mRNA Refseq: NM_130760
• Protein Refseq: NP_570116
• MIM: 102670
• UniProt ID: Q13477