Active Recombinant Human MADCAM1 protein, His-tagged
Cat.No. : | MADCAM1-3982H |
Product Overview : | Recombinant Human MADCAM1 protein(Gln 19 - Gln 317), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Human MAdCAM-1, His Tag immobilized on CM5 Chip can bind Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free with an affinity constant of 0.227 μM as determined in a SPR assay (Biacore 8K). |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 33.3 kDa. The protein migrates as 46-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Gln 19 - Gln 317 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. >95% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ] |
Official Symbol : | MADCAM1 |
Synonyms : | MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1; |
Gene ID : | 8174 |
mRNA Refseq : | NM_130760 |
Protein Refseq : | NP_570116 |
MIM : | 102670 |
UniProt ID : | Q13477 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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