Active Recombinant Human MAM Domain Containing Glycosylphosphatidylinositol Anchor 1, His-tagged

• Cat.No.: MDGA1-5686H
• Product Overview: Recombinant Human MDGA1protein was expressed in Murine myeloma cell line with a C-terminal 6-His tag. Gln19-Gly931.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 19-931 a.a.
• Description: MDGA1 is a 140 kDamember of the Ig superfamily of proteins. Human MDGA1 is synthesized as a 955amino acid (aa) precursor that contains an 18 aa signal sequence, a 914 aamature chain, and a 23 aa propeptide. The mature chain consists of sixIg-like domains, a fibronectin type III domain (aa 640-739), a MAM domain (aa751-918), 11 potential sites for N-linked glycosylation, and a GPI anchor (aa932). Alternative splicing in human MDGA1 produces an isoform variant thatshows a 48 aa substitution for aa corresponding to positions 926-955.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Gln19 predicted
• Molecular Weight: 101.9kDa
• Activity: Measuredby its ability to enhance neurite outgrowth of E18 rat embryonic corticalneurons. Able to significantly enhance neurite outgrowth when immobilized at7.5-30 μg/Ml on a nitrocellulose-coated microplate.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 120-140kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 500μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: MDGA1 MAM domain containingglycosylphosphatidylinositol anchor 1 [ Homo sapiens ]
• Official Symbol: MDGA1
• Synonyms: MDGA1; MAM domaincontaining glycosylphosphatidylinositol anchor 1; GPIM; MAMDC3; FLJ45018;DKFZp686K0262; DKFZp686L0262; MAM domain-containingglycosylphosphatidylinositol anchor protein 1; GPI and MAM protein; MAMdomain-containing protein 3; glycosylphosphatidylinositol-MAM;glycosyl-phosphatidyl-inositol-MAM; OTTHUMP00000016331; OTTHUMP00000016332
• Gene ID: 266727
• mRNA Refseq: NM_153487
• Protein Refseq: NP_705691
• MIM: 609626
• UniProt ID: Q8NFP4
• Chromosome Location: 6p21
• Function: molecular function