| Source : |
Baculovirus-Insect cells |
| Species : |
Human |
| Tag : |
His |
| Form : |
This product is not suitable for cell based experiments due to cytotoxicity of DDM.DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant. |
| Bio-activity : |
Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >35 pmol/min/μg. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 60.6 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE). |
| Protein Length : |
Ser 2 - Val 520 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
