Active Recombinant Human MAP1D protein, His-tagged

• Cat.No.: MAP1D-2201H
• Product Overview: Active Recombinant Human MAP1D protein(NP_954697.1) (Arg 44-Ala 335) was expressed in Insect Cells, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 44-335 aa
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0.
• Bio-activity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly--AMC. The resulting GP-AMC is cleaved by human DPPIV/CD26. The specific activity is >30 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human MAP1D consists of 303 amino acids and predicts a molecular mass of 33.4 kDa. It migrates as an apximately 36 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.