Active Recombinant Human MAP2K4, His-tagged

• Cat.No.: MAP2K4-105H
• Product Overview: Human MKK4, full length, amino acids M1-D399 (as in NCBI/Protein entry NP_003001.1), activated, N-terminal HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 1-399 a.a.
• Description: MAP2K4 encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. MAP2K4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
• Form: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
• Bio-activity: 41 pmol/μg×min
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles!
• Concentration: 0.884 μg/μl
• Full Length: Full L.

Gene Information

• Gene Name: MAP2K4 mitogen-activated protein kinase kinase 4 [ Homo sapiens ]
• Official Symbol: MAP2K4
• Synonyms: MAP2K4; mitogen-activated protein kinase kinase 4; SERK1; dual specificity mitogen-activated protein kinase kinase 4; JNKK1; MEK4; MKK4; PRKMK4; MEK 4; MAPKK 4; SAPK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK activating kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1; JNKK; SEK1; MAPKK4; SAPKK1; SAPKK-1
• Gene ID: 6416
• mRNA Refseq: NM_003010
• Protein Refseq: NP_003001
• MIM: 601335
• UniProt ID: P45985
• Chromosome Location: 17p12
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Ceramide signaling pathway, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity

Citation

Discovery of Covalent MKK4/7 Dual Inhibitor.

Journal: Cell chemical biology    PubMed ID: 32916088    Data: 2021/7/5

Authors: Jie Jiang, Baishan Jiang, Nathanael S Gray

Article Snippet:REAGENT or RESOURCE SOURCE IDENTIFIER Mycoalert Lonza Lonza Cat # LT07-318 Recombinant Human MKK7 protein, His & T7-tagged Creative BioMart Cat # MAP2K7-8083H Recombinant Human MKK4, His-tagged Creative BioMart Cat # MAP2K4-105H Anisomycin Santa Cruz Biotechnology Cat# SC-3524 CellTiter Glo Promega Cat # G7571 Critical Commercial Assays Lanthascreen (MKK4) Thermo Fisher Scientific Assay ID 2209 Lanthascreen (MKK4)_5 min Thermo Fisher Scientific Assay ID 2543 Lanthascreen (MKK4)_20 min Thermo Fisher Scientific Assay ID 2544 Lanthascreen (MKK4)_40 min Thermo Fisher Scientific Assay ID 2545 Lanthascreen (MKK4)_90 min Thermo Fisher Scientific Assay ID 2547 Z’ Lyte (GSK3a) Thermo Fisher Scientific Assay ID 492 Z’ Lyte (GSK3b) Thermo Fisher Scientific Assay ID 495 32P-ATP kinase assay (MKK7) Reaction Biology N/A Mobility shift (MKK7) NanoSyn N/A KiNativ kinase panel profiling AcitvX N/A KINOMEscan DiscoverX Corp. N/A BCA protein assay kit Thermo Fisher Scientific Cat # 23227 Deposited Data MKK4 protein structure Matsumoto, et al., 2010 PDB: 3ALN MKK2 protein structure Ohren, et al., 2004 PDB: 1S9I Experimental Models: Cell Lines Human: MDA-MB-231 cells (female) ATCC Cat # HTB-26; RRID:CVCL_0062 Recombinant DNA MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA Sigma Aldrich Cat # SHC016 Software and Algorithms Prism Graphpad RRID:SCR_002798 Other Streptavidin-agarose beads Thermo Fisher Cat # 20349