Active Recombinant Human MAPK13 protein, GST-tagged

• Cat.No.: MAPK13-357H
• Product Overview: Recombinant Human MAPK13 protein(O15264) (Met1-Leu365) was expressed in Insect Cells, fused with the GST tag at the N-terminus. Activated in vitro by MAP2K6.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-365 aa
• Form: Supplied as sterile 50 mM Tris-HCl, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol, pH 7.5.
• Bio-activity: The specific activity was determined to be 55 nmol/min/mg using MBP as substrate.
• Molecular Mass: The recombinant human MAPK13/GST chimera consists of 589 amino acids and predicts a molecular mass of 68.4 kDa. It migrates as an approximately 63.9 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MAPK13 mitogen-activated protein kinase 13 [ Homo sapiens ]
• Official Symbol: MAPK13
• Synonyms: MAPK13; mitogen-activated protein kinase 13; PRKM13; p38delta; SAPK4; MAPK 13; MAP kinase 13; MAP kinase p38 delta; stress-activated protein kinase 4; mitogen-activated protein kinase p38 delta; MGC99536
• Gene ID: 5603
• mRNA Refseq: NM_002754
• Protein Refseq: NP_002745
• MIM: 602899
• UniProt ID: O15264