Active Recombinant Human Matrix Metallopeptidase 2

• Cat.No.: MMP2-808H
• Product Overview: Recombinant Human MMP2 is expressed in Sf9-insect cells using the baculovirus expression vector system and purified from cell culture supernatant. The full-length recombinant proenzyme consists of 631 amino acids and has an apparent Mr of 72 kDa.

Specification

• Cat. No.: MMP2-808H
• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP"s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
• Source: Sf9-insect cell.
• Purity: Recombinant progelatinase A appears as a major band of 72 kDa in non-reducing SDS-PAGE (> 95 % of total protein). Due to autoproteolytic activity minor bands of activated gelatinase A (e.g. 62 or 50 kDa) may be visible in the enzyme preparation.
• Activity: The specific activity of gelatinase A after APMA activation is >120 mU/mg. 1 U is the activity that hydrolyzes 1 mmol peptide (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly- Leu-(3-[2,4-dinitrophenyl]-L-2,3-diaminopropionyl)- Ala-Arg-NH2 (Mca-Pro-Leu-G ly-Leu-D pa-Ala-Arg) within 1min under assay conditions described below.
• Application: Recombinant progelatinase A can serve as antigen standard in immunochemical analyses. Active gelatinase A may be used to study the degradation of extracellular matrix proteins, to screen inhibitors of matrix metalloproteinases and to characterize inhibitor actions.
• Storage: Recombinant MT1-MMP prodomain-catalytic domain is stable until the expiry date given on the label if stored at -70°C. It can be kept at -20 °C for several weeks. Repeated freezing and thawing should be avoided.
• Tag: Non

Gene Information

• Gene Name: MMP2 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) [ Homo sapiens ]
• Synonyms: MMP2; matrix metallopeptidase 2; CLG4, CLG4A, MMP-II, MONA, TBE-1; 72 kDa gelatinase; 72 kDa type IV collagenase; MMP-2; collagenase type IV-A; gelatinase A; matrix metalloproteinase-2; matrix metalloproteinase-II; neutrophil gelatinase; EC 3.4.24.24
• Gene ID: 4313
• mRNA Refseq: NM_001127891
• Protein Refseq: NP_001121363
• MIM: 120360
• UniProt ID: P08253
• Chromosome Location: 16q13-q21
• Pathway: Bladder cancer; GnRH signaling pathway; Leukocyte transendothelial migration; Diabetes pathways
• Function: metal ion binding; metalloendopeptidase activity; peptidase activator activity; sequence-specific DNA binding transcription factor activity; zinc ion binding

Reviews

06/19/2021

The solubility and ion exchange ability are very good, which is convenient for subsequent experimental treatment and purification.

05/13/2021

Kinetic parameters such as enzymatic reaction rate and binding affinity were tested in the early stage, and it was found that the protein performed well.

03/16/2021

MMP2 products undergo strict quality control to ensure the consistency and stability of each batch.

Q&As

How to regulate the activity of MMP2 in tumor therapy?

The activity of MMP2 is regulated by inhibitors and antibodies against it to inhibit tumor metastasis and invasion.

How is MMP2 related to metastases that promote disease progression?

MMP2 interacts with some transfer factors, such as VEGF and EGF, to promote tumor metastasis and invasion.

What are the application prospects of MMP2 inhibitors?

Inhibitors of MMP2 can be used as potential drug targets for the treatment of tumors, cardiovascular diseases and other related diseases.

Does MMP2 interact with the tumor microenvironment?

MMP2 interacts with tumor cells, immune cells, blood vessels, etc. in the tumor microenvironment to affect tumor progression and treatment effect.

What is the role of MMP2 in embryonic development?

It is involved in important processes such as morphogenesis, organ development and intraembryonic lymphatic vessel formation during embryonic development.

What are the effects of MMP2 mutations in human disease?

Mutations in MMP2 may affect its protein structure and function, leading to the occurrence and progression of related diseases.