Active Recombinant Human MELK, GST-tagged

• Cat.No.: MELK-1430H
• Product Overview: Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: MELK or maternal embryonic leucine zipper kinase is a member of the CAMKL kinase family. MELK is a key regulator of the proliferation of malignant brain tumors including their stem cells. MELK transcript abundance correlates with malignancy grade in human astrocytomas and represents a therapeutic target for the management of the most frequent brain tumors in adult and children. MELK also plays a role in mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL. Therefore, the kinase activity of MELK could be a promising molecular target for development of therapy for patients with breast cancers.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 200 nmol/min/mg
• Molecular Mass: ~61 kDa
• Protein length: 1-340 aa
• Purity: >85%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: MELK maternal embryonic leucine zipper kinase [ Homo sapiens ]
• Official Symbol: MELK
• Synonyms: MELK; maternal embryonic leucine zipper kinase; KIAA0175; pEg3 kinase; protein kinase Eg3; protein kinase PK38; tyrosine-protein kinase MELK; HPK38
• Gene ID: 9833
• mRNA Refseq: NM_001256685
• Protein Refseq: NP_001243614
• MIM: 607025
• UniProt ID: Q14680
• Chromosome Location: 9p13.1
• Function: ATP binding; calcium ion binding; lipid binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity