Active Recombinant Human Meprin A, Beta, His-tagged

• Cat.No.: MEP1B-1980H
• Product Overview: RecombinantHuman MEP1B protein was expressed in Murine myeloma cellline. Leu21-Ser593& Thr23-Ser593, both with a C-terminal 10-His tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 21-593;23-593 a.a.
• Description: Meprinsare multimeric proteases composed of α and β subunits, which are members ofthe astacin family of zinc endopeptidases. Both subunits form disulfide-linkedhomo-or eterooligomers, which are also referred to as Merpin A (composed of αsubunits with or without β subunits) and MEP1B (composedof β subunits only). Although the two subunits share 42% identity in theiramino acid sequence, they differ significantly in their oligomeric structure,posttranslational processing and subsequently cellular location, andsubstrate and peptide bond specificity.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and Glycerol.
• N-terminalSequence: Leu21 & Thr23
• MolecularWeight: 66 kDa
• Activity: Measuredby its ability to cleave a fluorogenic peptide substrate, McaSEVNLDAEFRK(Dpn)RR-NH2,The specific activity is > 350 pmoles/min/μg, as measured under thedescribed conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 85 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: MEP1B meprin A, beta [ Homosapiens ]
• Official Symbol: MEP1B
• Synonyms: MEP1B; meprin A,beta; meprin A subunit beta; PPH beta; meprin B; endopeptidase-2; PABApeptide hydrolase; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunitbeta; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; EC 3.4.24.63
• Gene ID: 4225
• mRNA Refseq: NM_005925
• Protein Refseq: NP_005916
• MIM: 600389
• UniProt ID: Q16820
• Chromosome Location: 18q12.2-q12.3
• Pathway: Protein digestion and absorption
• Function: metal ion binding; metalloendopeptidaseactivity; peptidase activity; zinc ion binding