| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
18-267 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Matrix Metalloproteinase 7 (MMP7) is a member of the matrix metalloproteinases (MMPs) family which are zinc and calcium dependent endopeptidases. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and can degrade a variety of extracellular matrix substrates and other substrates and plays important regulatory roles in many human pathophysiological processes.Besides,Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of MMP7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MMP7 and recombinant human MMP2. Briefly, biotin-linked MMP7 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to MMP2-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of MMP7 and MMP2 was shown, the EC50 for this effect is 0.30ug/mL. |
| Molecular Mass : |
29.5kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex. |
