Active Recombinant Human MSLN protein, His-Avi-tagged, Biotinylated

• Cat.No.: MSLN-0382H
• Product Overview: Biotinylated Recombinant Human MSLN protein(Glu296-Thr600), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Monoclonal Anti-Human MSLN Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-600), His,Avitag with a linear range of 0.1-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 37.8 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu296-Thr600
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421