Active Recombinant Human NCAM1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NCAM1-0549H
• Product Overview: Biotinylated Active Recombinant Human NCAM1 protein(P13591-2)(Leu20-Gly718) is expressed from HEK293 with His tag and Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-718 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human NCAM-1, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 80.29 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: NCAM1 neural cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: NCAM1
• Synonyms: NCAM1; neural cell adhesion molecule 1; CD56; NCAM; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal 5.1H11; MSK39
• Gene ID: 4684
• mRNA Refseq: NM_000615
• Protein Refseq: NP_000606
• MIM: 116930
• UniProt ID: P13591