Active Recombinant Human NCAM1 protein, His-tagged

• Cat.No.: NCAM1-591H
• Product Overview: Recombinant Human NCAM1 protein(Leu 20 - Gly 718), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu 20 - Gly 718
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH8.0, 10% trehalose.
• Bio-activity: Immobilized Human NCAM-1, His Tag at 1 μg/mL (100 μL/well) can bind Anti-NCAM-1 Antibody, Human IgG1 with a linear range of 0.6-10 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 79.3 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NCAM1 neural cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: NCAM1
• Synonyms: NCAM1; neural cell adhesion molecule 1; CD56; NCAM; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal 5.1H11; MSK39
• Gene ID: 4684
• mRNA Refseq: NM_000615
• Protein Refseq: NP_000606
• MIM: 116930
• UniProt ID: P13591