Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Met1-Gly135 |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized human NCR3 at 10μg/mL (100μL/well) can bind human B7-H6, the EC50 of human B7-H6 is 20-100ng/mL. |
Molecular Mass : |
The recombinant human NCR3 consists of 128 amino acids after removal of the signal peptide and has a predicted molecular mass of 14.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NCR3 is approximately 22-28 kDa due to glycosylation. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 97 % as determined by SDS-PAGE |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |