Active Recombinant Human NECTIN2 protein, His&Avi-tagged, Biotinylated

• Cat.No.: NECTIN2-3254H
• Product Overview: Biotinylated Recombinant Human NECTIN2 (NP_002847.1)(Met1-Leu360) was expressed in HEK293 with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 1-360 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human Nectin-2, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.