Species : |
Human |
Source : |
E.coli |
Tag : |
Non |
Description : |
Human NGF-beta is a neurotrophic factor related to BDNF, NT-3 and NT-4. Human NGF-beta acts as a neurotrophic factor through its receptor beta-NGFR, and is involved in the development and sustaining the sensory and sympathetic nervous systems. H-NGFbeta also is also involved in the growth and differentiation of B lymphocytes and B-cell survival. |
Form : |
Human NGF-beta is lyophilized with no additives. |
Purity : |
Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Bio-activity : |
Biological activity is determined by the ability to stimulate neurite growth in chick E9 DRG cells. The ED50 is < 1.0 ng/ml. |
Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility : |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 97% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Protein Content determined by : |
Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
Endotoxin Level : |
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
Amino acid sequence : |
MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRR |
Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
Pathways : |
Apoptosis, MAPK signaling pathway, Signalling by NGF |