Active Recombinant Human NLN protein, His-tagged

• Cat.No.: NLN-5693H
• Product Overview: Recombinant Human NLN protein(Ser38-Pro704), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 38-704 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH. The specific activity is > 100 pmol/min/μg.
• Molecular Mass: 78.7 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: NLN neurolysin (metallopeptidase M3 family) [ Homo sapiens ]
• Official Symbol: NLN
• Synonyms: NLN; neurolysin (metallopeptidase M3 family); AGTBP, angiotensin binding protein; neurolysin, mitochondrial; KIAA1226; endopeptidase 24.16; microsomal endopeptidase; neurotensin endopeptidase; angiotensin binding protein; angiotensin-binding protein; mitochondrial oligopeptidase M; MEP; MOP; AGTBP; EP24.16; FLJ23002; DKFZp564F123
• Gene ID: 57486
• mRNA Refseq: NM_020726
• Protein Refseq: NP_065777
• MIM: 611530
• UniProt ID: Q9BYT8