Active Recombinant Human Neurolysin (metallopeptidase M3 family), His-tagged

• Cat.No.: NLN-5693H
• Product Overview: Recombinant Human NLNprotein was expressed in E. coli with an N-terminal Met and 6-His tag. Ser38-Pro704.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 38-704 a.a.
• Description: NLN, also known asOligopeptidase M, soluble angiotensin II-binding protein and endopeptidase EC3.4.24.16, is a homologue of Thimet Ologopeptidase, a zinc peptidase of theM3 family that also includes mitochondrial intermediate peptidase. NLNexpresses in two forms with the difference of an N-terminal transit peptidethat targets the localization to mitochondria.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and Glycerol.
• N-terminalSequence: Met
• Molecular Weight: 77 kDa
• Activity: Measuredby its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Pro-D-Lys (2, 4-dinitrophenyl)-OH or Mca-PLGPK (Dnp)-OH.The specific activity is > 70 pmoles/min/μg, as measured under thedescribed conditions.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 73 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months fromdate of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C understerile conditions after opening.

Gene Information

• Gene Name: NLN neurolysin (metallopeptidaseM3 family) [ Homo sapiens ]
• Official Symbol: NLN
• Synonyms: NLN; neurolysin(metallopeptidase M3 family); MEP; MOP; AGTBP; EP24.16; FLJ23002; KIAA1226;DKFZp564F123; neurolysin, mitochondrial; endopeptidase 24.16; microsomalendopeptidase; neurotensin endopeptidase; angiotensin binding protein;angiotensin-binding protein; mitochondrial oligopeptidase M; EC 3.4.24.16; EC3.4.24; OTTHUMP00000123454; OTTHUMP00000222651
• Gene ID: 57486
• mRNA Refseq: NM_020726
• Protein Refseq: NP_065777
• MIM: 611530
• UniProt ID: Q9BYT8
• Chromosome Location: 5q12.3
• Pathway: Renin-angiotensin system
• Function: metal ion binding; metalloendopeptidaseactivity; peptidase activity