Recombinant Human NLN protein, His-tagged

• Cat.No.: NLN-348H
• Product Overview: Recombinant Human NLN protein(NP_065777.1) (Ser38-Pro704) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser38-Pro704
• Tag: N-His
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH. The specific activity is > 100 pmol/min/μg.
• Molecular Mass: The recombinant human NLN consists of 685 amino acids and predicts a molecular mass of 78.7 kDa. It migrates as an approximately 67 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NLN neurolysin (metallopeptidase M3 family) [ Homo sapiens ]
• Official Symbol: NLN
• Synonyms: NLN; neurolysin (metallopeptidase M3 family); AGTBP, angiotensin binding protein; neurolysin, mitochondrial; KIAA1226; endopeptidase 24.16; microsomal endopeptidase; neurotensin endopeptidase; angiotensin binding protein; angiotensin-binding protein; mitochondrial oligopeptidase M; MEP; MOP; AGTBP; EP24.16; FLJ23002; DKFZp564F123
• Gene ID: 57486
• mRNA Refseq: NM_020726
• Protein Refseq: NP_065777
• MIM: 611530
• UniProt ID: Q9BYT8