Active Recombinant Human Neurotensin Receptor 3/Sortilin

• Cat.No.: SORT1-15H
• Product Overview: Recombinant Human Neurotensin Receptor 3/Sortilin, expressed in Murine myeloma cell line.

Specification

• Description: Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10pD) family of sorting receptors.Human preprosortilin is pr centigradeessed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pHdependent high affinity binding that bl centigradeks the Sortilin ligand binding site both preand postcleavage. The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10 CC) essential for ligand binding. The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, end centigradeytosis and Golgiendosome transport? as little as 10% may be found on the cell surface.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rhSortilin at 4 μg/mL (100 μL/well) can bind rhβNGF with a linear range of 3-200 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 99.7 kDa.SDS PAGE: 95-100 kDa, reducing conditions.
• Endotoxin: <1.0 eu per 1 μg of the protein by the lal</1.0
• Purity: >97%, by SDSPAGE under reducing conditions and visualized by silver stain.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: 12 months from date of receipt, 20 to 70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, 20 to 70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SORT1 sortilin 1 [ Homo sapiens ]
• Official Symbol: SORT1
• Synonyms: sortilin; NTR3; glycoprotein 95; 100 kDa NT receptor; neurotensin receptor 3
• Gene ID: 6272
• mRNA Refseq: NM_001205228
• Protein Refseq: NP_001192157
• MIM: 602458
• UniProt ID: Q99523
• Chromosome Location: 1p13.3; 1p21.3-p13.1
• Pathway: BDNF signaling pathway, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome, conserved biosystem
• Function: enzyme binding; nerve growth factor binding; nerve growth factor receptor activity