Active Recombinant Human NFIC, GST-tagged

• Cat.No.: NFIC-325H
• Product Overview: The proline-rich activation domain of human CTF1 protein (amino acids 399-499) is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.

Specification

• Description: The CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator. It selectively activates transcription of genes that contain GCCAAT consensus sequence in the promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes. Cloning and functional characterization of the protein revealed that CTF1 contains a N-terminal DNA binding domain and a C-terminal proline-rich activation domain. CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes, with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene, as well as other receptor proteins to synergize hormone receptor-responsive activation.
• Source: E. coli
• Species: Human
• Tag: GST
• Bio-activity: 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
• Molecular Mass: 36.8 kDa.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: It can be applied in vitro dephosphorylation assay, DNA and protein-protein interactions assays.
• Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.
• Storage Buffer: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

Gene Information

• Gene Name: NFIC nuclear factor I/C (CCAAT-binding transcription factor) [ Homo sapiens ]
• Official Symbol: NFIC
• Synonyms: NFIC; nuclear factor I/C (CCAAT-binding transcription factor); NFI; nuclear factor 1 C-type; CTF; CTF5; NF I; NF1-C; NF-I/C; TGGCA-binding protein; CCAAT-box-binding transcription factor; NF-I; MGC20153
• Gene ID: 4782
• mRNA Refseq: NM_001245002
• Protein Refseq: NP_001157741
• MIM: 179617
• UniProt ID: Q06609
• Chromosome Location: 15q15.1
• Pathway: Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem
• Function: ATP binding; ATP binding; DNA-dependent ATPase activity; damaged DNA binding; double-stranded DNA binding; double-stranded DNA binding; identical protein binding; nucleoside-triphosphatase activity; nucleotide binding; protein C-terminus binding; protein binding; single-stranded DNA binding; single-stranded DNA-dependent ATPase activity