Active Recombinant Human NGF

Cat.No. : NGF-534H
Product Overview : Human NGF (P01138) full-elngth recombinant protein expressed in CHO cells.
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Species : Human
Source : Human
Tag : Non
Theoretical MW (kDa) : 16.95
Form : Lyophilized
Preparation Method : Mammalian cell (CHO) expression system
Purity : μ 98% by SDS-PAGE, μ 98% by HPLC
Endotoxin Level : < 0.1 ng/ug
Activity : The ED50 as determined using PC12 cell line is 1.2-2 ng/mL and using DRG cell line is 0.8-1.5 ng/mL.
Application : SDS-PAGE
Storage Buffer : Lyophilized from 30 mM sodium acetate, pH 5.5 (1% HAS)
Storage : Store at -20°C on dry atmosphere.
Unitprot ID : P01138
Pathways : Apoptosis, MAPK signaling pathway, Signalling by NGF
Gene Name NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
Official Symbol NGF
Synonyms Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; Beta-nerve growth factor precursor; beta-nerve growth factor; nerve growth factor, beta polypeptide; nerve growth factor, beta subunit;NGF_HUMAN; NGF; nerve growth factor (beta polypeptide)
Gene ID 4803
mRNA Refseq NM_002506
Protein Refseq NP_002497
MIM 162030
Chromosome Location 1p13.1
Function growth factor activity, nerve growth factor receptor binding, receptor signaling protein activity

Development of novel HDL-mimicking α-tocopherol-coated nanoparticles to encapsulate nerve growth factor and evaluation of biodistribution

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V    PubMed ID: 27531623    Data: 2016/8/12

Authors: Priyanka Prathipati, Jing Zhu, Xiaowei Dong

Article Snippet:Apo A–I was purchased from Athens research and technology (Athens, GA).Apo A–I was purchased from Athens research and technology (Athens, GA).. Recombinant human NGF was purchased from Creative BioMart (Shirley, NY).. Bradford reagent was obtained from thermo scientific (Rockford, IL).Bradford reagent was obtained from thermo scientific (Rockford, IL).

Non-helical DNA Triplex Forms a Unique Aptamer Scaffold for High Affinity Recognition of Nerve Growth Factor.

Journal: Structure (London, England : 1993)    PubMed ID: 26027732    Data: 2016/4/13

Authors: Thale C Jarvis, Douglas R Davies, Nebojsa Janjic

Article Snippet:The complex of SL1049 and recombinant human NGF (Creative BioMart, CHO cell expression) was crystallized at 16 C by combining 0.4 ml of proteinSOMAmer complex (0.33 mM in PBS buffer) with 0.4 ml of crystallization solution (14–16 % PEG 8000, 100 mM HEPES [pH 6.0], 100 mM magnesium acetate, and 20% glycerol) in a sitting drop vapor diffusion tray (Compact 300 96-well crystallization plates, Rigaku Reagents).. Sodium iodide was used for the heavy atom soak for phasing.Sodium iodide was used for the heavy atom soak for phasing.

Not For Human Consumption!

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