Active Recombinant Human NGF Protein (Ser122-Ala241), C-His tagged, Animal-free, Carrier-free

• Cat.No.: NGF-234H
• Product Overview: Recombinant Human NGF Protein (Ser122-Ala241) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Nerve Growth Factors (NGF) is critical for the development and maintenance of the sympathetic and sensory neuron systems. NGF has been demonstrated as a complex that consists of three polypeptides named α, β and γ subunits. Among then, β subunit, which known as beta-NGF is a 26.9 kDa protein containing 241 residues that involve in neuronal survival and differentiation. Besides, beta-NGF also acts as a ligand to TRKA receptor, which indispensable for the differentiation and development of pain and temperature sensing neurons.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.7 ng/mL. The specific activity of recombinant human beta-NGF is > 1 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 14.43 kDa.
  The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ser122-Ala241
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens (human) ]
• Official Symbol: NGF
• Synonyms: NGF; nerve growth factor (beta polypeptide); NGFB; beta-nerve growth factor; nerve growth factor, beta subunit; HSAN5; Beta-NGF; MGC161426; MGC161428
• Gene ID: 4803
• mRNA Refseq: NM_002506
• Protein Refseq: NP_002497
• MIM: 162030
• UniProt ID: P01138