Active Reombinant Human NDP Protein

• Cat.No.: NDP-489H
• Product Overview: Active Reombinant Human NDP Protein (25-133 aa) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 25-133 aa
• Description: This gene encodes a secreted protein with a cystein-knot motif that activates the Wnt/beta-catenin pathway. The protein forms disulfide-linked oligomers in the extracellular matrix. Mutations in this gene result in Norrie disease and X-linked exudative vitreoretinopathy.
• Form: Disulfide-linked oligomer, Lyophilized from a 0.2 μm filtered solution in HCl.
• Bio-activity: Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells expressing human Frizzled-4 and human LRP-5. The ED50 for this effect is 30-150 ng/mL.
• Molecular Mass: 12.6 kDa
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: > 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 250 μg/mL in sterile 4 mM HCl.

Gene Information

• Gene Name: NDP norrin cystine knot growth factor NDP [ Homo sapiens (human) ]
• Official Symbol: NDP
• Synonyms: NDP; norrin cystine knot growth factor NDP; ND; EVR2; FEVR; norrin; NDP, norrin cystine knot growth factor; Norrie disease (pseudoglioma); X-linked exudative vitreoretinopathy 2 protein; norrie disease protein
• Gene ID: 4693
• mRNA Refseq: NM_000266
• Protein Refseq: NP_000257
• MIM: 300658
• UniProt ID: Q00604