Active Recombinant Human NOTCH1 protein, Fc-tagged

• Cat.No.: NOTCH1-0569H
• Product Overview: Recombinant Human NOTCH1 protein(Ala19-Gln526), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 19-526 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DLL4, His Tag at 5 μg/mL (100 μL/well) can bind Human NOTCH1, Fc Tag with a linear range of 1-8 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 80.0 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala19-Gln526
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NOTCH1 notch 1 [ Homo sapiens ]
• Official Symbol: NOTCH1
• Synonyms: NOTCH1; notch 1; Notch (Drosophila) homolog 1 (translocation associated) , Notch homolog 1, translocation associated (Drosophila) , TAN1; neurogenic locus notch homolog protein 1; Notch homolog 1, translocation-associated; translocation-associated notch protein TAN-1; hN1; TAN1
• Gene ID: 4851
• mRNA Refseq: NM_017617
• Protein Refseq: NP_060087
• MIM: 190198
• UniProt ID: P46531