Active Recombinant Human NOTCH2 protein, Fc-tagged

• Cat.No.: NOTCH2-721H
• Product Overview: Recombinant Human NOTCH2(Q04721-1)(Leu 26 - Gln 530), fused to Fc Tag, is expressed from human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Leu 26 - Gln 530
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DLL4, His Tag at 5 μg/mL (100 μL/well) can bind Human NOTCH2, Fc Tag with a linear range of 5-78 ng/mL.
• Molecular Mass: The protein has a calculated MW of 80.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: NOTCH2 notch 2 [ Homo sapiens ]
• Official Symbol: NOTCH2
• Synonyms: NOTCH2; notch 2; Notch (Drosophila) homolog 2 , Notch homolog 2 (Drosophila); neurogenic locus notch homolog protein 2; Notch homolog 2; hN2; AGS2; HJCYS
• Gene ID: 4853
• mRNA Refseq: NM_001200001
• Protein Refseq: NP_001186930
• MIM: 600275
• UniProt ID: Q04721