Active Recombinant Human NOV protein, His-tagged

• Cat.No.: NOV-2211H
• Product Overview: Recombinant Human NOV protein(NP_002505.1)(Met1-Met357), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Met357
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10mM Imidazole, 10% Glycerol, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cells. When cells are added to CCN3-coated plates (10 μg/ml, 100 μl/well), >50% cells will adhere specifically after 60 minutes at 37 ℃.
• Molecular Mass: The secreted recombinant human NOV consists of 337 amino acids and predicts a molecular mass of 37 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NOV nephroblastoma overexpressed [ Homo sapiens ]
• Official Symbol: NOV
• Synonyms: NOV; nephroblastoma overexpressed; nephroblastoma overexpressed gene; protein NOV homolog; CCN3; IGFBP9; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog; NOVh; IBP-9; IGFBP-9
• Gene ID: 4856
• mRNA Refseq: NM_002514
• Protein Refseq: NP_002505
• MIM: 164958
• UniProt ID: P48745