| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
Tyr139-Thr257 |
| Form : |
Lyophilized from sterile PBS, pH 5.2. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 μg/ml (100 μl/well) can bind human TrkB-Fch. The EC50 of human TrkB-Fch is 120-350 ng/mL. |
| Molecular Mass : |
The recombinant human NT3 consists of 120 amino acids and has a predicted molecular mass of 13.8 kDa. The apparent molecular mass of it is approximately 16 kDa in SDS-PAGE under reducing conditions. |
| Purity : |
> 90 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
