Active Recombinant Human NTF3 Protein

Cat.No. : NT3-01H
Product Overview : Recombinant Human NTF3 Protein, Tyr139-Thr257 (K196R), without tag was expresssed in Spodoptera frugiperda, Sf 21 (baculovirus).
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  • Gene Information
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Description : The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Source : Insect cells
Species : Human
Bio-activity : Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 4-40 ng/mL.
The specific activity of Recombinant Human NT-3 is approximately 2082 U/μg, which is calibrated against recombinant human NT-3 Standard.
Molecular Mass : 13.7 kDa
Protein length : 139-257
N-terminal Sequence Analysis : Tyr139
Endotoxin : < 0.10 EU/μg of the protein by the LAL method.
Purity : >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution : Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping : At ambient temperature.
Gene Name : NTF3 neurotrophin 3 [ Homo sapiens (human) ]
Official Symbol : NTF3
Synonyms : NTF3; neurotrophin 3; NT3; HDNF; NGF2; NT-3; NGF-2; neurotrophin-3; nerve growth factor 2; neurotrophic factor
Gene ID : 4908
mRNA Refseq : NM_002527
Protein Refseq : NP_002518
MIM : 162660
UniProt ID : P20783

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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