Active Recombinant Human NTRK1 protein, His-tagged

• Cat.No.: NTRK1-977H
• Product Overview: Active Recombinant Human NTRK1 protein(Pro 194-Glu 413)(NP_002520.2), corresponding to the Ig-like C2-type 1 & 2 domains, was expressed and purified in HEK293, with a N-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 194-413 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit NGF-induced proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-4 μg/ml in the presence of 10 ng/mL of human NGF.
• Molecular Mass: The recombinant human NTRK1 Ig-like C2-type 1 & 2 domains (aa 194-413) consists of 239 amino acids and has a predicted molecular mass of 26 kDa. As a result of glycosylation, it migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001007793.1
• Unit ID: P04629
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P04629

Gene Information

• Gene Name: NTRK1 neurotrophic tyrosine kinase, receptor, type 1 [ Homo sapiens ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; MTC; TRK; TRKA; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein; TRK1; Trk-A; p140-TrkA; DKFZp781I14186
• Gene ID: 4914
• mRNA Refseq: NM_001007792
• protein Refseq: NP_001007793
• MIM: 191315