Active Recombinant Human OLFM2 Protein, His-tagged

• Cat.No.: OLFM2-802H
• Product Overview: Recombinant Human OLFM2 (Accession # NP_477512) Gln21-Pro454, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Gln21-Pro454
• Predicted N Terminal: No results obtained: Gln21 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells.When 5 x 10^4 cells per well are added to human Olfactomedin-2 coated plates (1 µg/mL, 100 µL/well), approximately 50% - 75% will adhere after 1 hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human OLFM2, His-tagged has a calculated MW of 50 kDa. In SDS-PAGE migrates as 55-75 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: OLFM2 olfactomedin 2 [ Homo sapiens ]
• Official Symbol: OLFM2
• Synonyms: NOE2; OlfC; NOELIN2; NOELIN2_V1
• Gene ID: 93145
• mRNA Refseq: NM_058164.2
• Protein Refseq: NP_477512.1
• UniProt ID: O95897
• Chromosome Location: 19p13.2