Active Recombinant Human OTUD6B, GST-tagged

• Cat.No.: OTUD6B-179H
• Product Overview: Recombinant human OTUD3 (amino acid residues 1-629), fused with N-terminal GST, was expressed in E.coli.

Specification

• Description: OTUD6B is a cysteine protease and is a member of the OTU (ovarian tumour) superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Kayagaki et al. (2007). OTU enzymes play important roles as negative-feedback regulators in NF-κB signalling, interferon signalling and in p97 (cdc48)-mediated processes although the cellular functions of most OTU enzymes remain to be discovered.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-OTUD6B was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-OTUD6B was compared confirming the deubiquitylating activity of GST-OTUD6B.
• Molecular Mass: ~64 kDa
• Purity: >98% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: OTUD6B OTU domain containing 6B [ Homo sapiens ]
• Official Symbol: OTUD6B
• Synonyms: OTUD6B; OTU domain containing 6B; OTU domain-containing protein 6B; CGI 77; DUBA5; DUBA-5; CGI-77
• Gene ID: 51633
• mRNA Refseq: NM_016023
• Protein Refseq: NP_057107
• MIM: 612021
• UniProt ID: Q8N6M0
• Chromosome Location: 8q21.3