Active Recombinant Human P2RX7 Full Length Transmembrane protein(Nanodisc)

• Cat.No.: P2RX7-1219H
• Product Overview: Recombinant Human P2RX7 Full Length Transmembrane protein(Q99572), was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Bio-activity: Elisa plates were pre-coated with Flag Tag P2RX7-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with P2RX7-Nanodisc is 5.349ng/ml.
• Molecular Mass: The human full length P2RX7 protein has a MW of 68.4 kDa.
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Full Length: Full L.

Gene Information

• Gene Name: P2RX7 purinergic receptor P2X, ligand-gated ion channel, 7 [ Homo sapiens ]
• Official Symbol: P2RX7
• Synonyms: P2RX7; purinergic receptor P2X, ligand-gated ion channel, 7; P2X purinoceptor 7; MGC20089; P2X7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• Gene ID: 5027
• mRNA Refseq: NM_002562
• Protein Refseq: NP_002553
• MIM: 602566
• UniProt ID: Q99572

Q&As

I am interested on P2X7 insertion on arificial bilayer for single channel functional measurements. Do you think this product would fit?

To perform single channel functional measurements on the P2X7 protein, there are several requirements that need to be considered: 1. Purification of the P2X7 protein: The P2X7 protein needs to be purified to ensure that only the target protein is inserted into the artificial bilayer membrane for measurements. This typically involves expression and purification of the protein from a suitable expression system such as mammalian cells or bacteria. 2. Protein stability: The P2X7 protein needs to be stable in order to maintain its structure and function during the insertion process and measurements. Proper handling and storage conditions should be maintained to prevent protein degradation. 3. Protein concentration: The concentration of the P2X7 protein should be optimized for successful insertion into the artificial bilayer membrane. Too low of a concentration may result in insufficient protein insertion, while too high of a concentration may lead to non-specific binding or aggregation. Based on the above factors, we suppose P2RX7-1219H is a suitable protein for your application. This full length protein is very stable even at RT and its purity is greater than 90%. It is in lyophilized form and you can resolve it at your ideal concentration.