Active Recombinant Human P4HB protein, His-tagged

• Cat.No.: P4HB-310H
• Product Overview: Active Recombinant Human P4HB protein(NP_000909.2)(Met 1-Lys 505) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-505 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human PH4B consists of 499 amino acids and has a predicted molecular mass of 56.4 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 60 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: P4HB prolyl 4-hydroxylase, beta polypeptide [ Homo sapiens ]
• Official Symbol: P4HB
• Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; ERBA2L, PO4DB, procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide , procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide (protein disulfide isomerase associated 1) , procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); protein disulfide-isomerase; collagen prolyl 4 hydroxylase beta; DSI; GIT; P4Hbeta; PDI; PDIA1; PO4HB; PROHB; protein disulfide isomerase family A; member 1; protein disulfide isomerase associated 1; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase family A, member 1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PHDB; PO4DB; ERBA2L
• Gene ID: 5034
• mRNA Refseq: NM_000918
• Protein Refseq: NP_000909
• MIM: 176790
• UniProt ID: P07237