Active Recombinant Human PDCD1 protein, His-tagged
Cat.No. : | PDCD1-2173H |
Product Overview : | Recombinant Human PDCD1 protein(NP_005009.2)(Met1-Gln167), fused with His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile 50mM MOPS, 500mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | 1. Immobilized human PD-1 at 2 μg/ml (100 μl/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of human B7-H1 is 150-600 ng/mL. 2. Immobilized human PD-1 at 2 μg/mL (100 μL/well) can bind Anti-PDCD1(MDX)-IgG4 Antibody (Nivolumab), the EC50 is 8-60 ng/mL.3. Labeled biotin to PD-1 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (His Tag), Biotinylated bound to Keytruda was 0.1 nM. |
Molecular Mass : | The recombinant mature human PDCD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PDCD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Gln167 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Recombinant Protein | ||
PDCD1-699M | Recombinant Mouse PDCD1 Protein, Fc-tagged | +Inquiry |
PDCD1-1173H | Recombinant Human PDCD1 Protein, His-tagged | +Inquiry |
PDCD1-1222R | Active Recombinant Rhesus PDCD1 protein(Met1-Gln167), His-tagged | +Inquiry |
PDCD1-22H | Active Recombinant Human PDCD1 Protein, Fc-tagged, Alexa Fluor® 647 conjugated | +Inquiry |
PDCD1-625M | Recombinant Mouse PDCD1 Protein, DDK/His-tagged | +Inquiry |
◆ Lysates | ||
PDCD1-2628MCL | Recombinant Mouse PDCD1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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