PDE3B-1526H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human PDE3B (654-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description :
PDE3B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE3B has high affinity for cAMP and is inhibited by cGMP as well as specific inhibitors such as cilostamide and milrinone. PDE3B is phosphorylated and activated in response to insulin and agents that increase cAMP. Leptin can increase the activity of PDE3B in the hypothalamus leading to a decrease in cAMP concentration. PDE3B-knockout mice exhibited multiple alterations in regulation of lipolysis, lipogenesis, and insulin secretion, as well as signs of peripheral insulin resistance.
Source :
Sf9 Cells
Species :
Human
Tag :
GST
Form :
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity :
156 nmol/min/mg
Molecular Mass :
~85 kDa
Protein length :
654 aa-end
Purity :
>70%
Applications :
PDE Assay
Storage :
Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.