Active Recombinant Human PDE6A, GST-tagged

• Cat.No.: PDE6A-1613H
• Product Overview: Recombinant human PDE6A (31-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 38 nmol/min/mg
• Molecular Mass: ~120 kDa
• Protein length: 31-end
• Purity: >70%
• Applications: PDE Assay
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PDE6A phosphodiesterase 6A, cGMP-specific, rod, alpha [ Homo sapiens ]
• Official Symbol: PDE6A
• Synonyms: PDE6A; phosphodiesterase 6A, cGMP-specific, rod, alpha; PDEA; rod cGMP-specific 3,5-cyclic phosphodiesterase subunit alpha
• Gene ID: 5145
• mRNA Refseq: NM_000440
• Protein Refseq: NP_000431
• MIM: 180071
• UniProt ID: P16499
• Chromosome Location: 5q31.2-q34
• Pathway: Activation of the phototransduction cascade; Inactivation, recovery and regulation of the phototransduction cascade; Signal Transduction
• Function: 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding