Active Recombinant Human PDPK1, GST-tagged

• Cat.No.: PDPK1-561H
• Product Overview: Recombinant Human PDPK1, full length with N-terminal GST tag, MW=89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Specification

• Description: In the field of biochemistry, 3-phosphoinositide dependent protein kinase-1, also known as PDPK1 is a protein which in humans is encoded by thePDPK1 gene. It is implicated in the development and progression of melanomas.
• Source: Baculovirus infected Sf9 cell
• Species: Human
• Tag: GST
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT.
• Bio-activity: 5.02 pmol/min/μg Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80°C

Gene Information

• Gene Name: PDPK1 3-phosphoinositide dependent protein kinase-1 [ Homo sapiens (human) ]
• Official Symbol: PDPK1
• Synonyms: PDPK1; PDK1; PDPK2; PRO0461; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; PkB kinase like gene 1; NP_001248745.1; EC 2.7.11.1; NP_002604.1
• Gene ID: 5170
• mRNA Refseq: NM_002613
• Protein Refseq: NP_002604
• MIM: 605213
• UniProt ID: O15530
• Chromosome Location: 16p13.3
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events; Activation of PKB; Adaptive Immune System
• Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding