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Active Recombinant Human PDPK1, GST-tagged

Cat.No. : PDPK1-561H
Product Overview : Recombinant Human PDPK1, full length with N-terminal GST tag, MW=89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
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Description : In the field of biochemistry, 3-phosphoinositide dependent protein kinase-1, also known as PDPK1 is a protein which in humans is encoded by thePDPK1 gene. It is implicated in the development and progression of melanomas.
Source : Baculovirus infected Sf9 cell
Species : Human
Tag : GST
Form : 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT.
Bio-activity : 5.02 pmol/min/μg Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.
Applications : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Stability : >6 months at –80°C
Gene Name : PDPK1 3-phosphoinositide dependent protein kinase-1 [ Homo sapiens (human) ]
Official Symbol : PDPK1
Synonyms : PDPK1; PDK1; PDPK2; PRO0461; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; PkB kinase like gene 1; NP_001248745.1; EC 2.7.11.1; NP_002604.1
Gene ID : 5170
mRNA Refseq : NM_002613
Protein Refseq : NP_002604
MIM : 605213
UniProt ID : O15530
Chromosome Location : 16p13.3
Pathway : Activation of NMDA receptor upon glutamate binding and postsynaptic events; Activation of PKB; Adaptive Immune System
Function : 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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