Active Recombinant Human PDPN protein, Fc-His-tagged

• Cat.No.: PDPN-2320H
• Product Overview: Active Recombinant Human PDPN protein(Q86YL7-1) (Met 1-Lys 123) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-123 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.4-1.0 μg/mL.
• Molecular Mass: The recombinant human PDPN/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 349 amino acids with a predicted molecular mass of 38.6 kDa. As a result of glycosylation, rh PDPN/Fc monomer migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001006625.1
• Unit ID: Q86YL7
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q86YL7

Gene Information

• Gene Name: PDPN podoplanin [ Homo sapiens ]
• Official Symbol: PDPN
• Synonyms: PDPN; podoplanin; aggrus; Gp38; GP40; lung type I cell membrane associated glycoprotein; PA2.26; T1A 2; T1-alpha; hT1alpha-1; hT1alpha-2; PA2.26 antigen; glycoprotein 36; glycoprotein, 36-KD; lung type-I cell membrane-associated glycoprotein (T1A-2); T1A; GP36; OTS8; T1A-2; AGGRUS; HT1A-1
• Gene ID: 10630
• mRNA Refseq: NM_001006624
• protein Refseq: NP_001006625
• MIM: 608863