Active Recombinant Human PDPN, Fc-His tagged

• Cat.No.: PDPN-2320H
• Product Overview: Recombinant Human PDPN (Q86YL7-1) extracellular domain (Met 1-Lys 123), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Lys123
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.4-1.0 μg/mL.
• Molecular Mass: The recombinant human PDPN/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 349 amino acids with a predicted molecular mass of 38.4 kDa. As a result of glycosylation, rh PDPN/Fc monomer migrates as an approximately 50.2 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PDPN podoplanin [ Homo sapiens ]
• Official Symbol: PDPN
• Synonyms: PDPN; podoplanin; aggrus; Gp38; GP40; lung type I cell membrane associated glycoprotein; PA2.26; T1A 2; T1-alpha; hT1alpha-1; hT1alpha-2; PA2.26 antigen; glycoprotein 36; glycoprotein, 36-KD; lung type-I cell membrane-associated glycoprotein (T1A-2); T1A; GP36; OTS8; T1A-2; AGGRUS; HT1A-1
• Gene ID: 10630
• mRNA Refseq: NM_001006624
• Protein Refseq: NP_001006625
• MIM: 608863
• UniProt ID: Q86YL7