Active Recombinant Human PH20 protein

• Cat.No.: PH20-1889H
• Product Overview: Recombinant Human PH20 protein(Leu 36 - Ser 490) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: Leu 36 - Ser 490
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture).
• Molecular Mass: This protein carries no tag. The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.