Active Recombinant Human PI3 kinase (p110α/p85α) protein, GST-tagged

• Cat.No.: PIK3CA-1894H
• Product Overview: Recombinant Human PI3 kinase (p110α/p85α) fused with N-terminal GST-tag(p110α), no tag on p85α, was co-expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT.
• Bio-activity: 880 pmol/min/μg
• Molecular Mass: 235 kDa (complex); p110α MW=151 kDa, p85α MW=84 kDa.
• Unit Definition: A unit of p110α/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37 centigrade.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 centigrade. Avoid freeze/thaw cycles

Gene Information

• Gene Name: PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide [ Homo sapiens ]
• Official Symbol: PIK3CA
• Synonyms: PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; p110-alpha; MGC142161; MGC142163
• Gene ID: 5290
• mRNA Refseq: NM_006218
• Protein Refseq: NP_006209
• MIM: 171834
• UniProt ID: P42336
• Chromosome Location: 3q26.3
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol 3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; protein kinase activator activity; protein serine/threonine kinase activity