Active Recombinant Human PIK3CA/PIK3R3 protein, His-tagged

Cat.No. : PIK3CA&PIK3R3-152H
Product Overview : Recombinant full-length human PI3K (p110 alpha/p55 gamma) was co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
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Description : PI3K (p110 alpha/p55 gamma) or Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which serve as second messengers in growth signaling pathways. PI3K comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in the PI3K has been associated with insulin resistance and plays an important role in glucose homeostasis in vivo. PI3K also plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses.
Source : Insect cells
Species : Human
Tag : His
Form : 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Bio-activity : 420 nmol/min/mg
Molecular Mass : p110 alpha ~111 kDa and p55 gamma ~61 kDa
Purity : >70%
Applications : Kinase Assay
Stability : 1 year at -70 centigrade from the date of shipment
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1μg/μl
SDS-PAGE:
Gene Name : PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide [ Homo sapiens ]
Official Symbol : PIK3CA
Synonyms : PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; p110-alpha; MGC142161; MGC142163;
Gene ID : 5290
mRNA Refseq : NM_006218
Protein Refseq : NP_006209
MIM : 171834
UniProt ID : P42336
Chromosome Location : 3q26.3
Pathway : 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem;
Function : 1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol 3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; protein kinase activator activity; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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